The effects of α-lactalbumin and whey protein concentrate on α-amino acids, calcium and phosphorus levels in blood and gastrointestinal tract of rats

نویسندگان

  • Odile T. PANTAKO
  • Jean AMIOT
چکیده

The effects of two dietary proteins on α-amino acids, calcium and phosphorus concentrations in plasma, stomach and intestine were investigated in rats trained to consume, in a single two-hour daily meal, diets containing α-lactalbumin (α-la) or whey protein concentrate (WPC) for two weeks. The results indicated that the concentrations of calcium and phosphorus in the gastrointestinal tract and that of α-amino acids in portal vein were not significantly influenced by the nature of diets. The amount of α-amino acids in the gastrointestinal tract of rats fed on WPC diet was significantly (p < 0.001) higher than that of α-la group. The levels of insoluble calcium and insoluble phosphorus in the small intestine were significantly (p < 0.001) higher in α-la group than in WPC group. These results indicated that the kinetics of α-amino acids, calcium and phosphorus were differently influenced by the nature of diet ingested, the sampling time and the sites of sample collections. α-lactalbumin / whey protein / α-amino acid / calcium phosphorus / digestion Résumé — Effets de l’α-lactalbumine et d’un concentré de lactosérum sur les teneurs en acides α-aminés, en calcium et en phosphore dans le plasma et le tube digestif chez les rats. Les effets de protéines alimentaires sur les teneurs en acides α-aminés, en calcium et en phosphore dans le plasma et le tube digestif ont été étudiés chez des rats alimentés pendant deux semaines selon des régimes contenant de l’α-lactalbumine (α-la) ou un concentré de lactosérum (WPC). Les concentrations de calcium et de phosphore solubles dans le tube digestif et celles des acides α-aminés dans la veine porte, étaient comparables pour les deux groupes étudiés. En revanche, la quantité d’acides α-aminés dans le tube digestif des rats du groupe WPC était significativement (p < 0,001) supérieure. Les niveaux de calcium et de phosphore insoluble dans le tube digestif des rats du groupe α-la étaient significativement (p < 0,001) plus élevés. Ces résultats montrent que les profils des acides α-aminés, du calcium et du phosphore solubles dans le tube digestif étaient différemment influencés par la nature protéique du régime ingéré, le temps et les sites d’échantillonnage. α-lactalbumine / protéines de lactosérum / acide α-aminé / calcium / phosphore Reprod. Nutr. Dev. 41 (2001) 227–238 227 © INRA, EDP Sciences, 2001 * Correspondence and reprints E-mail: [email protected] O.T. Pantako, J. Amiot 228

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تاریخ انتشار 2001